Journal of Archives in Military Medicine

Published by: Kowsar

Subcloning and Expression of Leishmania major (MRHO/IR/75/ER) P4 Gene

Minoo Shaddel 1 , Hormozed Oormazdi 2 , * , Lame Akhlaghi 2 , Bahram Kazemi 3 , 4 and Mojgan Bandehpour 3 , 4
Authors Information
1 Department of Medical Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, IR Iran
2 Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
3 Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
4 Molecular and Cellular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Article information
  • Journal of Archives in Military Medicine: September 01, 2013, 1 (1); 19-23
  • Published Online: September 24, 2013
  • Article Type: Research Article
  • Received: August 6, 2013
  • Revised: September 3, 2013
  • Accepted: September 6, 2013
  • DOI: 10.5812/jamm.13809

To Cite: Shaddel M, Oormazdi H, Akhlaghi L, Kazemi B, Bandehpour M. Subcloning and Expression of Leishmania major (MRHO/IR/75/ER) P4 Gene, J Arch Mil Med. 2013 ; 1(1):19-23. doi: 10.5812/jamm.13809.

Copyright © 2013, AJA University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Carrillo E, Ahmed S, Goldsmith-Pestana K, Nieto J, Osorio Y, Travi B, et al. Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis. Vaccine. 2007; 25(8): 1534-43[DOI][PubMed]
  • 2. Markell EK, John DT, Krotoski WA. Med Parasitol. 1999; : 148-59
  • 3. Shaddel M. Expression of Leishmania major p4 gene. . 2008;
  • 4. Shi W, Schramm VL, Almo SC. Nucleoside hydrolase from Leishmania major. Cloning, expression, catalytic properties, transition state inhibitors, and the 2.5-a crystal structure. J Biol Chem. 1999; 274(30): 21114-20[PubMed]
  • 5. Leprohon P, Legare D, Girard I, Papadopoulou B, Ouellette M. Modulation of Leishmania ABC protein gene expression through life stages and among drug-resistant parasites. Eukaryot Cell. 2006; 5(10): 1713-25[DOI][PubMed]
  • 6. Miller TK, Patel C, Selitrennikoff CP. Construction of a Saccharomyces cerevisiae strain expressing the Leishmania major nucleoside hydrolase gene. Int J Antimicrob Agents. 2007; 29(1): 103-7[DOI][PubMed]
  • 7. Igarashi M, Honda S, Yamazaki J, Miyachi Y, Morishita T, Otuka Y, et al. [A case of Weber-Christian disease with elevated angiotensin-converting enzyme (ACE) activity and myopathy]. Nihon Naika Gakkai Zasshi. 1989; 78(11): 1599-600[PubMed]
  • 8. Moosa-Kazemi SH, Yaghoobi-Ershadir MR, Akhavan AA, Abdoli H, Zahraei-Ramazani AR, Jafari R, et al. Deltamethrin-impregnated bed nets and curtains in an anthroponotic cutaneous leishmaniasis control program in northeastern Iran. Ann Saudi Med. 2007; 27(1): 6-12[PubMed]
  • 9. Fazaeli A, Fouladi B, Sharifi I. Emergence of cutaneous leishmaniasis in a border area at south-east of Iran: an epidemiological survey. J Vector Borne Dis. 2009; 46(1): 36-42[PubMed]
  • 10. Ogbunude PO, Lamour N, Barrett MP. Molecular cloning, expression and characterization of ribokinase of Leishmania major. Acta Biochim Biophys Sin (Shanghai). 2007; 39(6): 462-6[PubMed]
  • 11. Soong L, Duboise SM, Kima P, McMahon-Pratt D. Leishmania pifanoi amastigote antigens protect mice against cutaneous leishmaniasis. Infect Immun. 1995; 63(9): 3559-66[PubMed]
  • 12. Stober CB, Lange UG, Roberts MT, Gilmartin B, Francis R, Almeida R, et al. From genome to vaccines for leishmaniasis: screening 100 novel vaccine candidates against murine Leishmania major infection. Vaccine. 2006; 24(14): 2602-16[DOI][PubMed]
  • 13. Pan AA, McMahon-Pratt D. Monoclonal antibodies specific for the amastigote stage of Leishmania pifanoi. I. Characterization of antigens associated with stage- and species-specific determinants. J Immunol. 1988; 140(7): 2406-14[PubMed]
  • 14. Campbell K, Diao H, Ji J, Soong L. DNA immunization with the gene encoding P4 nuclease of Leishmania amazonensis protects mice against cutaneous Leishmaniasis. Infect Immun. 2003; 71(11): 6270-8[PubMed]
  • 15. Kar S, Soong L, Colmenares M, Goldsmith-Pestana K, McMahon-Pratt D. The immunologically protective P-4 antigen of Leishmania amastigotes. A developmentally regulated single strand-specific nuclease associated with the endoplasmic reticulum. J Biol Chem. 2000; 275(48): 37789-97[DOI][PubMed]
  • 16. Shaddel M, Oormazdi H, Akhlaghi L, Kazemi B, Bandehpour M. Cloning of Leishmania major p4 Gene. Yakhteh Med J. 2008; 10(3): 201-4
  • 17. Kar S, Metz C, McMahon-Pratt D. CD4+ T cells play a dominant role in protection against New World leishmaniasis induced by vaccination with the P-4 amastigote antigen. Infect Immun. 2005; 73(6): 3823-7[DOI][PubMed]
  • 18. Rott MB, Fernandez V, Farias S, Ceni J, Ferreira HB, Haag KL, et al. Comparative analysis of two different subunits of antigen B from Echinococcus granulosus: gene sequences, expression in Escherichia coli and serological evaluation. Acta Trop. 2000; 75(3): 331-40[PubMed]
  • 19. Sambrook J, Russell DW. Molecular Cloning. A laboratory manual. 1989;
  • 20. Singh N, Nagar S, Roy U, Agrawal SS, Agrawal S, Rastogi AK. Antigenic analysis of soluble fraction of Leishmania donovani by SDS-polyacrylamide gel electrophoresis and immunoblotting. Indian J Exp Biol. 1995; 33: 313-15
  • 21. Farajnia S, Alimohammadian MH, Reiner NE, Karimi M, Ajdari S, Mahboudi F. Molecular characterization of a novel amastigote stage specific Class I nuclease from Leishmania major. Int J Parasitol. 2004; 34(8): 899-908[DOI][PubMed]
  • 22. Farajnia S, Mahboudi F, Ajdari S, Reiner NE, Kariminia A, Alimohammadian MH. Mononuclear cells from patients recovered from cutaneous leishmaniasis respond to Leishmania major amastigote class I nuclease with a predominant Th1-like response. Clin Exp Immunol. 2005; 139(3): 498-505[DOI][PubMed]
  • 23. Taghipour N, Bandepour M, Pazoki R, Haghighi A, Nazari Pouya MR, Kazemi B. Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in pQE-30 Expression Vector. Iran J Parasitol. 2009; 4(4): 1-9
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments