Detection of blaSPM-1 Metallo-?-Lactamase Gene in Imipenem-Resistant Pseudomonas aeruginosa Strains Isolated From Hospitalized Patients in Isfahan Hospitals

AUTHORS

Mansour Sedighi 1 , Amir Hasanzadeh 2 , Saeid Safiri 3 , Naeema Syedi 4 , Shayan Mostafaei 5 , Jamshid Faghri 6 , *

1 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran

2 Division of Microbiology, Department of Pathobiology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, IR Iran

3 Department of Public Health, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, IR Iran

4 School of Pharmacy and Medical Sciences, Sansom Institute for Health Research, University of South Australia, Adelaide, Australia

5 Department of Epidemiology and Biostatistics, School of Public Health, Isfahan University of Medical Sciences, Isfahan, IR Iran

6 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran

How to Cite: Sedighi M, Hasanzadeh A, Safiri S, Syedi N, Mostafaei S, et al. Detection of blaSPM-1 Metallo-?-Lactamase Gene in Imipenem-Resistant Pseudomonas aeruginosa Strains Isolated From Hospitalized Patients in Isfahan Hospitals, J Arch Mil Med. 2015 ; 3(2):26977. doi: 10.5812/jamm.3(2)2015.26977.

ARTICLE INFORMATION

Journal of Archives in Military Medicine: 3 (2); 26977
Published Online: May 29, 2015
Article Type: Research Article
Received: January 13, 2015
Accepted: February 15, 2015
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Abstract

Background: Pseudomonas aeruginosa is an opportunistic human pathogen, which causes serious problems especially in people who have immunodeficiency. Recently, metallo-?-lactamase (MBLs) resistance in this bacterium has led to some difficulties in treating bacterial infections. The blaSPM-1 is one of the MBL gene families, which induces resistance to the carbapenem class antibiotics; this gene has not been previously assessed in Iran.

Objectives: Detection and quantification of blaSPM-1- metallo-?-lactamase gene among resistant Pseudomonas aeruginosa strains (imipenem), isolated from patients in Isfahan hospitals.

Patients and Methods: A total of 180 samples were isolated from various nosocomial infections. These isolates were identified as Pseudomonas aeruginosa by using biochemical tests. In order to determine their bacterial drug resistance-pattern the Kirby-Bauer disk diffusion method was utilized. Presence of MBLs in imipenem isolates was detected using the combine disk technique (IMP-EDTA). Similarly, an E-test on Mueller-Hinton agar was used to determine the minimal inhibitory concentration (MIC) of imipenem isolates. The imipenem isolates were then subjected to polymerase chain reaction (PCR) to detect the blaSPM-1 gene. Data were analyzed using the SPSS software (version 16, SPSS Inc., Chicago, IL, USA).

Results: In total, 96 isolates of Pseudomonas aeruginosa were collected. Of all isolates, 34 (35.41%) were found to be imipenem-resistant P. aeruginosa. The MIC levels in all imipenem-resistant strains were MIC ? 32 ?g/mL. Thirteen (38.23%) of the imipenem-resistant P. aeruginosa isolates were MBL positive. None of the isolates carried the blaSPM-1 gene, as indicated by the PCR assay.

Conclusions: The rate of imipenem resistance due to MBL has increased dramatically. Early detection and infection-control practices are the best antimicrobial strategy for this organism.

Keywords

Pseudomonas aeruginosa Imipenem Nosocomial Infection blaSPM-1 Metallo-?-Lactamase

© 2015, Journal of Archives in Military Medicine. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

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