Journal of Archives in Military Medicine

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Effect of High Intensity Interval Training with Flaxseed on Interleukin-1 Beta and Lipocalin-2 Gene Expressions in the Heart Tissue of Rats

Younes Khademi 1 , * , Seyed Ali Hosseini 2 , Fatemeh Dana 3 , Azar Hamidi 4 , Majid Azadmanesh 5 and Mehdi Pirouz 5
Authors Information
1 Department of Physical Education, University of Science and Art, Yazd, Iran
2 Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
3 Student of Medicine, Yazd Branch, Islamic Azad University, Yazd, Iran
4 Department of Physical Education, Bardaskan Branch, Islamic Azad University, Bardaskan, Iran
5 Department of Physical Education, Bam Branch, Islamic Azad University, Bam, Iran
Article information
  • Journal of Archives in Military Medicine: 6 (4); e83888
  • Published Online: January 2, 2019
  • Article Type: Research Article
  • Received: September 3, 2018
  • Revised: December 21, 2018
  • Accepted: December 23, 2018
  • DOI: 10.5812/jamm.83888

To Cite: Khademi Y , Hosseini S A, Dana F , Hamidi A, Azadmanesh M , et al. Effect of High Intensity Interval Training with Flaxseed on Interleukin-1 Beta and Lipocalin-2 Gene Expressions in the Heart Tissue of Rats, J Arch Mil Med. Online ahead of Print ; 6(4):e83888. doi: 10.5812/jamm.83888.

Copyright © 2019, Journal of Archives in Military Medicine. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Kargarfard M, Lam ET, Shariat A, Asle Mohammadi M, Afrasiabi S, Shaw I, et al. Effects of endurance and high intensity training on ICAM-1 and VCAM-1 levels and arterial pressure in obese and normal weight adolescents. Phys Sportsmed. 2016;44(3):208-16. doi: 10.1080/00913847.2016.1200442. [PubMed: 27291761].
  • 2. Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, et al. Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clin Chem. 2007;53(1):34-41. doi: 10.1373/clinchem.2006.075614. [PubMed: 17040956].
  • 3. Yan QW, Yang Q, Mody N, Graham TE, Hsu CH, Xu Z, et al. The adipokine lipocalin 2 is regulated by obesity and promotes insulin resistance. Diabetes. 2007;56(10):2533-40. doi: 10.2337/db07-0007. [PubMed: 17639021].
  • 4. Zhang J, Wu Y, Zhang Y, Leroith D, Bernlohr DA, Chen X. The role of lipocalin 2 in the regulation of inflammation in adipocytes and macrophages. Mol Endocrinol. 2008;22(6):1416-26. doi: 10.1210/me.2007-0420. [PubMed: 18292240]. [PubMed Central: PMC2422824].
  • 5. Kralisch S, Weise S, Sommer G, Lipfert J, Lossner U, Bluher M, et al. Interleukin-1beta induces the novel adipokine chemerin in adipocytes in vitro. Regul Pept. 2009;154(1-3):102-6. doi: 10.1016/j.regpep.2009.02.010. [PubMed: 19233230].
  • 6. Sommer G, Weise S, Kralisch S, Lossner U, Bluher M, Stumvoll M, et al. Lipocalin-2 is induced by interleukin-1beta in murine adipocytes in vitro. J Cell Biochem. 2009;106(1):103-8. doi: 10.1002/jcb.21980. [PubMed: 19009554].
  • 7. Bahmani P, Halabian R, Masroori N, Rouhbakhsh M, Ebrahimi M, Nourani MR, et al. [Induction of heme oxygenase-1 by lipocalin 2 mediated by NF-κB transcription factor]. J Iran Anat Sci. 2009;7:33-44. Persian.
  • 8. Mohammadi Karizno F, Saghebjoo M, Foadoddini M, Sarir H. [The role of aerobic training and Pistacia atlantica extract on the levels of protein carbonyl, heat shock protein 70, and glycogen in the liver tissue of diabetic rats]. J Birjand Univ Med Sci. 2014;21(1):35-47. Persian.
  • 9. Zuk M, Kulma A, Dyminska L, Szoltysek K, Prescha A, Hanuza J, et al. Flavonoid engineering of flax potentiate its biotechnological application. BMC Biotechnol. 2011;11:10. doi: 10.1186/1472-6750-11-10. [PubMed: 21276227]. [PubMed Central: PMC3040132].
  • 10. Sneddon AA, Tsofliou F, Fyfe CL, Matheson I, Jackson DM, Horgan G, et al. Effect of a conjugated linoleic acid and omega-3 fatty acid mixture on body composition and adiponectin. Obesity (Silver Spring). 2008;16(5):1019-24. doi: 10.1038/oby.2008.41. [PubMed: 18356842].
  • 11. Mirfatahi M, Tabibi H, Nasrollahi A, Hedayati M, Taghizadeh M. Effect of flaxseed oil on serum systemic and vascular inflammation markers and oxidative stress in hemodialysis patients: A randomized controlled trial. Int Urol Nephrol. 2016;48(8):1335-41. doi: 10.1007/s11255-016-1300-5. [PubMed: 27115157].
  • 12. Ren GY, Chen CY, Chen GC, Chen WG, Pan A, Pan CW, et al. Effect of flaxseed intervention on inflammatory marker C-reactive protein: A systematic review and meta-analysis of randomized controlled trials. Nutrients. 2016;8(3):136. doi: 10.3390/nu8030136. [PubMed: 26959052]. [PubMed Central: PMC4808865].
  • 13. Khademi Y, Azarbayjani MA, Hosseini SA. [Simultaneous effect of high-intensity interval training (HIIT) and consumption of flaxseed on serum levels of TNF-α and IL1β in rats]. Horizon Med Sci. 2017;23(4):257-63. Persian.
  • 14. Khademi Y, Azarbayejani MA, Hossini A. [The effect of high intensity aerobic interval training (HIIT) and flaxseed oil on ICAM- 1 gene expression in heart tissue of male wistar rats]. Armaghan Danesh. 2016;21(116):873-86. Persian.
  • 15. Beavers KM, Brinkley TE, Nicklas BJ. Effect of exercise training on chronic inflammation. Clin Chim Acta. 2010;411(11-12):785-93. doi: 10.1016/j.cca.2010.02.069. [PubMed: 20188719]. [PubMed Central: PMC3629815].
  • 16. Talanian JL, Galloway SD, Heigenhauser GJ, Bonen A, Spriet LL. Two weeks of high-intensity aerobic interval training increases the capacity for fat oxidation during exercise in women. J Appl Physiol (1985). 2007;102(4):1439-47. doi: 10.1152/japplphysiol.01098.2006. [PubMed: 17170203].
  • 17. Lovatel GA, Elsner VR, Bertoldi K, Vanzella C, Moyses Fdos S, Vizuete A, et al. Treadmill exercise induces age-related changes in aversive memory, neuroinflammatory and epigenetic processes in the rat hippocampus. Neurobiol Learn Mem. 2013;101:94-102. doi: 10.1016/j.nlm.2013.01.007. [PubMed: 23357282].
  • 18. Agarwal D, Welsch MA, Keller JN, Francis J. Chronic exercise modulates RAS components and improves balance between pro- and anti-inflammatory cytokines in the brain of SHR. Basic Res Cardiol. 2011;106(6):1069-85. doi: 10.1007/s00395-011-0231-7. [PubMed: 22124756]. [PubMed Central: PMC3261080].
  • 19. Jenkins NT, Padilla J, Arce-Esquivel AA, Bayless DS, Martin JS, Leidy HJ, et al. Effects of endurance exercise training, metformin, and their combination on adipose tissue leptin and IL-10 secretion in OLETF rats. J Appl Physiol (1985). 2012;113(12):1873-83. doi: 10.1152/japplphysiol.00936.2012. [PubMed: 23019312]. [PubMed Central: PMC3544496].
  • 20. Molanouri Shamsi M, Hassan ZH, Gharakhanlou R, Quinn LS, Azadmanesh K, Baghersad L, et al. Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: Effect of resistance exercise training. Endocrine. 2014;46(1):60-9. doi: 10.1007/s12020-013-0038-4. [PubMed: 24006180].
  • 21. Mansouri M, Nikooie R, Keshtkar A, Larijani B, Omidfar K. Effect of endurance training on retinol-binding protein 4 gene expression and its protein level in adipose tissue and the liver in diabetic rats induced by a high-fat diet and streptozotocin. J Diabetes Investig. 2014;5(5):484-91. doi: 10.1111/jdi.12186. [PubMed: 25411614]. [PubMed Central: PMC4188104].
  • 22. Hasanvand B, Soori R, Rastegar Moghadam Mansouri M, Abbasian S. [The effect of high intensity interval training on gene expression of inflammatory biomarkers in fast-and slow muscles of rats]. Armaghane Danesh. 2017;21(12):1164-78. Persian.
  • 23. Pak Rad B, Agha Alinejad H, Zamani A, Fashi M, Rezaei Seraji B, Rajabi Z. [Study of effect of aerobic exercise in particulate air pollution tlr4 and il-1β genes expression in male wistar rats’heart tissue]. Sport Physiol. 2016;8(31):77-92. Persian.
  • 24. Nounou HA, Deif MM, Shalaby MA. Effect of flaxseed supplementation and exercise training on lipid profile, oxidative stress and inflammation in rats with myocardial ischemia. Lipids Health Dis. 2012;11:129. doi: 10.1186/1476-511X-11-129. [PubMed: 23036047]. [PubMed Central: PMC3508923].
  • 25. Christian F, Smith EL, Carmody RJ. The Regulation of NF-kappaB Subunits by Phosphorylation. Cells. 2016;5(1). doi: 10.3390/cells5010012. [PubMed: 26999213]. [PubMed Central: PMC4810097].
  • 26. Choi KM, Kim TN, Yoo HJ, Lee KW, Cho GJ, Hwang TG, et al. Effect of exercise training on A-FABP, lipocalin-2 and RBP4 levels in obese women. Clin Endocrinol (Oxf). 2009;70(4):569-74. doi: 10.1111/j.1365-2265.2008.03374.x. [PubMed: 18710473].
  • 27. Bijeh N, Abbasian S. [The effect of intensity of aerobic training and dietary pattern changing on interleukin-1β and resistance insulin indexes in non-active obese subjects]. Arak Med Univ J. 2013;16(7):1-13. Persian.
  • 28. Speisman RB, Kumar A, Rani A, Foster TC, Ormerod BK. Daily exercise improves memory, stimulates hippocampal neurogenesis and modulates immune and neuroimmune cytokines in aging rats. Brain Behav Immun. 2013;28:25-43. doi: 10.1016/j.bbi.2012.09.013. [PubMed: 23078985]. [PubMed Central: PMC3545095].
  • 29. Yang Y, Lv J, Jiang S, Ma Z, Wang D, Hu W, et al. The emerging role of Toll-like receptor 4 in myocardial inflammation. Cell Death Dis. 2016;7. e2234. doi: 10.1038/cddis.2016.140. [PubMed: 27228349]. [PubMed Central: PMC4917669].
  • 30. Dange RB, Agarwal D, Masson GS, Vila J, Wilson B, Nair A, et al. Central blockade of TLR4 improves cardiac function and attenuates myocardial inflammation in angiotensin II-induced hypertension. Cardiovasc Res. 2014;103(1):17-27. doi: 10.1093/cvr/cvu067. [PubMed: 24667851].
  • 31. Halder SK, Sharan C, Al-Hendy A. 1,25-dihydroxyvitamin D3 treatment shrinks uterine leiomyoma tumors in the Eker rat model. Biol Reprod. 2012;86(4):116. doi: 10.1095/biolreprod.111.098145. [PubMed: 22302692]. [PubMed Central: PMC3338660].
  • 32. Sallam N, Laher I. Exercise modulates oxidative stress and inflammation in aging and cardiovascular diseases. Oxid Med Cell Longev. 2016;2016:7239639. doi: 10.1155/2016/7239639. [PubMed: 26823952]. [PubMed Central: PMC4707375].
  • 33. Yu XH, Zheng XL, Tang CK. Nuclear factor-kappaB activation as a pathological mechanism of lipid metabolism and atherosclerosis. Adv Clin Chem. 2015;70:1-30. doi: 10.1016/bs.acc.2015.03.004. [PubMed: 26231484].
  • 34. Bagul PK, Deepthi N, Sultana R, Banerjee SK. Resveratrol ameliorates cardiac oxidative stress in diabetes through deacetylation of NFkB-p65 and histone 3. J Nutr Biochem. 2015;26(11):1298-307. doi: 10.1016/j.jnutbio.2015.06.006. [PubMed: 26298192].
  • 35. Steven S, Munzel T, Daiber A. Exploiting the pleiotropic antioxidant effects of established drugs in cardiovascular disease. Int J Mol Sci. 2015;16(8):18185-223. doi: 10.3390/ijms160818185. [PubMed: 26251902]. [PubMed Central: PMC4581241].
  • 36. Yin K, Agrawal DK. Vitamin D and inflammatory diseases. J Inflamm Res. 2014;7:69-87. doi: 10.2147/JIR.S63898. [PubMed: 24971027]. [PubMed Central: PMC4070857].
  • 37. Chen S, Swier VJ, Boosani CS, Radwan MM, Agrawal DK. Vitamin D deficiency accelerates coronary artery disease progression in swine. Arterioscler Thromb Vasc Biol. 2016;36(8):1651-9. doi: 10.1161/ATVBAHA.116.307586. [PubMed: 27255724]. [PubMed Central: PMC4965317].
  • 38. Norman PE, Powell JT. Vitamin D and cardiovascular disease. Circ Res. 2014;114(2):379-93. doi: 10.1161/CIRCRESAHA.113.301241. [PubMed: 24436433].
  • 39. Mohebbi H, Moghadasi M, Rahmani-Nia F, Hassan-Nia S, Noroozi H. [Effect of 12 weeks life-style activity modification (LAM) on adiponectin gene expression and plasma adiponectin in obese men]. Iran J Endocrinol Metab. 2010;12(1):25-81. Persian.
  • 40. de Graaf-Roelfsema E, Keizer HA, van Breda E, Wijnberg ID, van der Kolk JH. Hormonal responses to acute exercise, training and overtraining. A review with emphasis on the horse. Vet Q. 2007;29(3):82-101. doi: 10.1080/01652176.2007.9695232. [PubMed: 17970286].
  • 41. Graham TE, Yang Q, Bluher M, Hammarstedt A, Ciaraldi TP, Henry RR, et al. Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects. N Engl J Med. 2006;354(24):2552-63. doi: 10.1056/NEJMoa054862. [PubMed: 16775236].
  • 42. Chung HY, Cesari M, Anton S, Marzetti E, Giovannini S, Seo AY, et al. Molecular inflammation: Underpinnings of aging and age-related diseases. Ageing Res Rev. 2009;8(1):18-30. doi: 10.1016/j.arr.2008.07.002. [PubMed: 18692159]. [PubMed Central: PMC3782993].
  • 43. Gomes EC, Silva AN, de Oliveira MR. Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species. Oxid Med Cell Longev. 2012;2012:756132. doi: 10.1155/2012/756132. [PubMed: 22701757]. [PubMed Central: PMC3372226].
  • 44. Visioli F, Giordano E, Nicod NM, Davalos A. Molecular targets of omega 3 and conjugated linoleic Fatty acids - "micromanaging" cellular response. Front Physiol. 2012;3:42. doi: 10.3389/fphys.2012.00042. [PubMed: 22393325]. [PubMed Central: PMC3289952].
  • 45. Richard D, Bausero P, Schneider C, Visioli F. Polyunsaturated fatty acids and cardiovascular disease. Cell Mol Life Sci. 2009;66(20):3277-88. doi: 10.1007/s00018-009-0085-4. [PubMed: 19590823].

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